Composite

Part:BBa_K2593006:Design

Designed by: Huimin Li   Group: iGEM18_SSTi-SZGD   (2018-09-21)


PlepA-RBS-AmyX -6His-LHyal-T1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 561


Design Notes

Source

PlepA (BBa_K823002) ，The strong ribosome binding site (RBS) is a shine-Dalgarno sequence from gsiB gene. AmyX signal peptide is encoded by a-Amylase gene which is derived from B.amyloliquefaciens.